Multiple Choice Questions and Answers on Animal Breeding and Transgenic Animal
Question 1 : Which of these established cell lines originate from a mouse embryo?
- 3T3
- BHK
- HeLa
- BTK
Answer : 1
Question 2 : The nucleous of mature unfertilized ovum may be removed by
- irradiation
- surgery
- both (1) and (2)
- neutralization and homogenization
Answer : 3
Question 3 : Karyoplast is
- cells devoid of cell wall
- nuclei
- nuclei with only some residual plasma membrane
- cell with nucleus
Answer : 3
Question 4 : The success of embryo transplantation depends upon the ability to obtain an increased number of embryos from animals of superior genetic merit, which can be achieved by the use of
- exogenous gonadatrophic hormones
- endogenous gonadatrophic hormones
- both (1) and (2)
- FSH (Follicle Stimulating Hormone)
Answer : 1
Question 5 : Multiple ovulation and embryo transfer (MOET)
- can increase the rate of progress in dairy cattle
- allows progeny testing of males
- allows progeny testing of females
- all of the above
Answer : 4
Question 6 : The technique, mainly used for the diagnosing birth defects in the fetus by means of needle, is called
- amniocentesis
- ectogensis
- transplantation
- all of the above
Answer : 1
Question 7 : DNA is microinfected into the fertilized egg
- after the fusion of male and female nuclei
- before the fusion of male and female nuclei
- at the time of fusion of male and female nuclei
- any time, it can be infected
Answer : 2
Question 8 : In which year, Dolly the first mammalian clone was born?
- 1995
- 1997
- 1996
- 1998
Answer : 3
Question 9 : Which of the following statements is false?
- Continuous variation produces a spread of variation within a population
- Discontinuous variation is the result of genetics alone
- Blood group is an example of discontinuous variation
- Discontinuous variation is influenced by the environment
Answer : 4
Question 10 : Embryo transfer (ET) refers to the technique by which fertilized ova are collected from the reproductive tract of a genetically
- superior female and transferred to the inferior
- inferior female and transferred to the superior
- balanced female
- none of the above
Answer : 1
Question 11 : The number of follicles at any particular stage is governed by
- the rate of entry of dormant follicles
- the rate of growth of follicles
- the rate of loss of follicles
- all of the above
Answer : 4
Question 12 : Enucleation of the cells can be achieved by treating the cells with
- polyethylene glycol
- cytochalasin B
- both (1) and (2)
- alcohol
Answer : 2
Question 13 : When the cells are enucleated and centrifuged, nuclei
- detach from cell and settle at the bottom
- detach from cell and remain in the supernatant
- nuclei remain attached to the cell
- none of the above
Answer : 1
Question 14 : Superovulation is an
- increased ovulatory response by external hormonal therapy
- decreased ovulatory response by external hormonal therapy
- increased ovulatory response by internal hormonal therapy
- decreased ovulatory response by internal hormonal therapy
Answer : 1
Question 15 : Which of the following best describes artificial insemination?
- Transplanting an embryo into the uterus
- Fertilization of an egg in a test tube
- Selectively breeding healthy animals
- Taking the sperm and placing it directly
Answer : 4
Question 16 : Which part of a cell carries the information that is passed from one generation to the next?
- Cell
- Nucleus
- Cytoplasm
- Chloroplast
Answer : 2
Question 17 : Cloning is a method by which numbers of genetically identical organisms are derived from a single organism by
- vegetative propagation
- vegetative initiation
- vegetative termination
- none of these
Answer : 1
Question 18 : Which of the following is/are the method of transfection for making transgenic animals?
- Transfer of whole nuclei
- Transfer of whole individual chromosomes or fragment
- Transfer of DNA
- All of the above
Answer : 4
Question 19 : DNA microinjection into the egg has been used to produce which of the following transgenic animals?
- Mice
- Chicken
- Pigs
- All of these
Answer : 4
Question 20 : Transgenic goats produce a variant of human tissue type plasminogen activator protein in
- blood
- urine
- milk
- muscles
Answer : 3
Question 21 : For the rapid genetic progress, embryo transfer (ET)
- will be as potent a tool as AI (artificial insemination)
- will not be as potent a tool as AI (artificial insemination)
- ET can’t be used at all
- will be a superior tool than AI
Answer : 2
Question 22 : Which of the following statements best describes a clone?
- An artificial life form
- An offspring where all of the genetic material in every cell is identical to that of both parents
- An offspring where all of the genetic material in every cell is identical to that of one of its parents
- A type of sheep
Answer : 3
Question 23 : In transgenic fish, the genes are introduced by
- microinjection in fish
- viruses
- transfer of whole nuclei
- all of these
Answer : 1
Question 24 : Production of transgenic animals require transfections of
- eggs or embryos
- stem cells
- red blood cells
- all of these
Answer : 1
Question 25 : Which of the following statement is correct?
- Variation is caused by genes
- Variation is caused by the environment
- Variation can be caused by both genes and the environment
- None of the above
Answer : 1
Question 26 : Which of the following gene have been introduced into the transgenic fish?
- Human or rat gene for growth hormone
- Chicken gene for delta crystalline protein
- E. coli gene for β-galactosidase
- All of the above
Answer : 4
Question 27 : Which of these carries the thousands of genes needed to allow animal and plant cells to pass on inherited characteristics?
- Chromosomes
- Chromatogram
- Chromatography
- Chromium
Answer : 1
Question 28 : Animal pharming can be defined as
- growing animals for farming
- programming animals to produce novel products
- generating transgenic animals for farming
- none of the above
Answer : 2
Question 29 : When a fertilized egg cell develops into an embryo, the entropy of the living system
- decreases
- increase
- remains constant
- none of these
Answer : 1
Question 30 : For the transfer of whole individual chromosomes, they are isolated from the cells at
- prophase
- metaphase
- telophase
- anaphase
Answer : 2
Question 31 : Fusion of karyoplast with the enucleated cell is achieved in presence of
- cytochalasin B
- polyethylene glycol
- both (1) and (2)
- alcohol
Answer : 2
Question 32 : Transgenic goats have been used to produce which of the following protein that is used for dissolving blood clots?
- Amyloid precursor protein
- α1-anti trypsin (AAT)
- Casein
- A variant of human tissue-type plasminogen activator
Answer : 4
Question 33 : Transfection refers to which of the following?
- Synthesis of mRNA from DNA template
- Synthesis of protein based on mRNA sequence
- Introduction of foreign gene in to a cell
- The process by which a cell become malignant
Answer : 3
Question 34 : Chromosomes may be isolated from metaphase cells by
- hypertonic lysis
- hypotonic lysis
- either (1) or (2)
- isotonic lysis
Answer : 2
Question 35 : Superovulation is primarily the result of
- decreased atresia in medium and large follicles (>1.7 mm)
- increased atresia in medium and large follicles (>1.7 mm)
- increased atresia in the large follicles (<1.7 mm)
- decreased atresia in small follicles (<1.7 mm)
Answer : 1
Question 36 : Genetic change can occur only in
- one way
- two ways
- three ways
- four ways
Answer : 4
Question 37 : Some types of variation are due to changes in the genetic material. What is this type of change called?
- Fertilisation
- Mutation
- Radiation
- Sterilisation
Answer : 2
Question 38 : Which of the following is the process of choosing parent organisms for the characteristics that is wanted in their offspring?
- Active selection
- Reproductive selection
- Selective breeding
- Breeding selection
Answer : 3
Question 39 : Which protein has been produced generating a transgenic sheep that is used for replacement therapy for individuals at risk from emphysema?
- Plasminogen activator (tPA)
- α-anti trypsin (AAT)
- Casein
- Amyloid precursor proteins
Answer : 2
Question 40 : Which of the following is the word used to describe what happens when the nucleus of a sperm joins with the nucleus of an egg cell?
- Sex
- Fertilisation
- Intercourse
- Reproduction
Answer : 2
Question 41 : DNA into fish is injected into
- pronuclei
- cytoplasm
- both (1) and (2)
- none of these
Answer : 2