Antibiotics Questions and Answers

Multiple Choice Questions and Answers on Antibiotics

Question 1 : Which of the following changes occur during first phase of growth of Penicillium chrysogenum?

  1. Growth of mycelia occurs
  2. Ammonia is liberated in the medium
  3. Lactic acid present in the corn steep liquor is utilized at the maximum rate
  4. All of the above

Answer : 4

Question 2 : Which of the following event occurs during second phase of growth of P. chrysogenum?

  1. Synthesis of penicillin is high
  2. Mycelial mass increases
  3. Both (1) and (2)
  4. pH increase

Answer : 3

Question 3 : Inoculum preparation for the fermentation medium for penicillin takes place on

  1. wheat seeds
  2. barley seeds
  3. rice seeds
  4. all of these

Answer : 2

Question 4 : Antibiotics are typically produced in fed batch reactors because

  1. antibiotic yields are generally higher when cells enter the stationary phase
  2. the precursors are often toxic to the cells
  3. antibiotic yields are generally higher when cell growth slows
  4. All of the above

Answer : 4

Question 5 : Which of the following event occurs during third phase of growth of P chrysogenum?

  1. Concentration of antibiotic increases in the medium
  2. Autolysis of the medium starts
  3. Slight rise in pH due to liberation of ammonia
  4. All of the above

Answer : 4

Question 6 : Vegetable oil, (corn oil or soybean oil) added in the fermentation medium for penicillin, act as

  1. source of nutrients
  2. carbon source
  3. antifoam agent
  4. both (1) and (3)

Answer : 3

Question 7 : Which of the following organism(s) produce(s) tetracycline?

  1. Steptomyces aureofaciens
  2. Steptomyces ramosus
  3. Nocardia sulphurea
  4. All of these

Answer : 4

Question 8 : Media used for the production of chlorotetracyclin consists of

  1. sugar, corn steep liquor, CaCO3, (NH4)2SO4 and NH4 Cl
  2. corn steep liquor, CaCO3 and (NH4)2SO4
  3. sugar, CaCO3 and (NH4)2SO4
  4. corn steep liquor, CaCO3, (NH4)2SO4 and NH4 Cl

Answer : 1

Question 9 : Which of the following precursor is added in the medium to get penicillin G?

  1. Phenyl carbamic acid
  2. Phenyl acetic acid
  3. Ammonium sulphate
  4. Ammonium chloride

Answer : 2

Question 10 : High yield of chlorotetracyclin requires

  1. no aeration
  2. continuous aeration
  3. aeration which does not affect the yield
  4. controlled aeration

Answer : 2

Question 11 : Streptomycin is produced by

  1. S. griseus
  2. S. griseoflavus
  3. S. aerofaciens
  4. S. ramosus

Answer : 1

Question 12 : At acidic pH, penicillin remains in

  1. aqueous phase
  2. solvent phase
  3. both (1) and (2)
  4. precipitates

Answer : 2

Question 13 : Chlorotetracyclin is soluble in

  1. organic solvents
  2. water
  3. ether
  4. all of these

Answer : 2

Question 14 : Penicillin is produced by

  1. aerobic fermentation
  2. anaerobic fermentation
  3. aerobic fermentation followed by anaerobic fermentation
  4. anaerobic fermentation followed by aerobic fermentation

Answer : 1

Question 15 : Penicillin is recovered after fermentation as

  1. potassium penicillin
  2. calcium penicillin
  3. sodium penicillin
  4. penicillin only

Answer : 1

Question 16 : Media composition for the production of streptomycin is

  1. soybean meal, glucose, peptone, malt extract, sodium chloride
  2. soybean meal, glucose, peptone, malt extract, ammonium chloride
  3. soybean meal, glucose, peptone, malt extract, calcium carbonate
  4. soybean meal, glucose, peptone, malt extract, ammonium sulphate

Answer : 1

Question 17 : The yield of the antibiotic depends upon

  1. pH of the medium
  2. age of the inoculum
  3. composition of the medium
  4. all of these

Answer : 4

Question 18 : During which phase of growth of Penicillium chrysogenum maximum antibiotic production takes place

  1. during the first phase
  2. during the second phase
  3. during the third phase
  4. same in all the phases

Answer : 2

Question 19 : Fermentation medium for oxytetracyclin (terramycin) consist of

  1. CSL, starch, (NH4)2 SO4, sodium chloride and CaCO3
  2. CSL, (NH4)2 SO4, sodium chloride and CaCO3
  3. CSL, starch,(NH4)2 SO4, ammonium chloride and CaCO3
  4. CSL,(NH4)2 SO4, ammonium chloride and CaCO3

Answer : 1

Question 20 : pH of the fermentation medium for chlorotetracyclins is

  1. 5-6
  2. 6-7
  3. 7-8
  4. 8-9

Answer : 1

Question 21 : At normal pH, penicillin remains in

  1. aqueous phase
  2. solvent phase
  3. both (1) and (2)
  4. precipitates

Answer : 1