Cloning Vectors Questions and Answers

Multiple Choice Questions and Answers on Cloning Vectors

Question 1 : Cosmids lack

  1. genes coding for viral proteins
  2. origin of replication
  3. marker genes coding for replication
  4. cleavage site for the insertion of foreign DNA

Answer : 1

Question 2 : Cryptic plasmids

  1. do not exhibit any phenotypic trait
  2. exhibit many phenotypic traits
  3. exhibit one phenotypic traits
  4. exhibit antibiotic resistance

Answer : 1

Question 3 : Phagemid consist of

  1. plasmid vector carrying λ phage’s cos site
  2. plasmid vector carrying λ attachment (λ att) site
  3. plasmid vector carrying origin of replication of λ phage only
  4. plasmid vector carrying origin of replication of plasmid only

Answer : 2

Question 4 : Maximum size of foreign DNA that can be inserted into an insertion vector is

  1. 35 kb
  2. 18 kb
  3. 50 kb
  4. 27 kb

Answer : 2

Question 5 : Plasmids which are maintained as multiple copy number per cell are known as

  1. stringent plasmids
  2. relaxed plasmids
  3. cryptic plasmids
  4. none of these

Answer : 2

Question 6 : Cosmid vectors are

  1. plasmids that contain fragment of λ DNA including the cos site
  2. phages that lack cos site
  3. plasmids that have no selection marker
  4. cryptic plasmids

Answer : 1

Question 7 : EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to

  1. 6-7 kb
  2. 15-25 kb
  3. 40-44 kb
  4. 1-2 kb

Answer : 2

Question 8 : Size of the DNA that can be packaged into a λ phage is

  1. 50 kb
  2. 35-53 kb
  3. 40-50 kb
  4. any size

Answer : 2

Question 9 : Charon 34 and Charon 35 are the examples of

  1. plasmid vector
  2. cosmid vector
  3. phage vector
  4. phagemid vector

Answer : 3

Question 10 : Cosmid vectors are used for

  1. cloning small fragments of DNA
  2. cloning large fragments of DNA
  3. cloning prokaryotic DNA only
  4. cloning eukaryotic DNA only

Answer : 2

Question 11 : Plasmids which are maintained as limited number of copies per cell are known as

  1. stringent plasmids
  2. relaxed plasmids
  3. cryptic plasmids
  4. all of these

Answer : 1

Question 12 : Cos site of the cosmids

  1. consists of 12 bases
  2. helps whole genome in circularization and ligation
  3. both (1) and (2)
  4. contains cleavage site

Answer : 3

Question 13 : M 13 is an example of

  1. filamentous phage
  2. single stranded DNA vector
  3. both (1) and (2)
  4. plasmid

Answer : 3

Question 14 : Phagemid vectors are

  1. combination of plasmid and phage λ
  2. combination of phages and cosmid
  3. phages carrying properties of plasmids
  4. all of the above

Answer : 1

Question 15 : Single stranded vectors are useful

  1. for sequencing of cloned DNA
  2. for oligonucleotide directed mutagenesis
  3. for probe preparation
  4. all of the above

Answer : 4

Question 16 : Inserted DNA in λ gt 11 can be expressed as

  1. β-galactosidase fused protein
  2. free protein in the cytoplasm
  3. free protein that is secreted out
  4. all of the above

Answer : 1

Question 17 : Plasmid incompatibility is

  1. inability of a plasmid to grow in the host
  2. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
  3. both (1) and (2)
  4. none of the above

Answer : 2

Question 18 : P1 cloning vector allow cloning of DNA of the length of

  1. 100 kbp
  2. 50 kbp
  3. 20 kbp
  4. 10 kbp

Answer : 1

Question 19 : Charon 34 and Charon 35 can clone DNA upto

  1. 1-2 kb
  2. 6-7 kb
  3. 9-20 kb
  4. 30-35 kb

Answer : 3

Question 20 : Charon vectors are different from EMBL vectors because

  1. they have more extensive range of restriction targets with in their polylinkers
  2. physical separation of lambda arm from central fragment is required
  3. both (1) and (2)
  4. physical separation of lambda arm from central fragment is not required

Answer : 3

Question 21 : P1 cloning vector is the example of

  1. plasmid
  2. cosmid
  3. bacteriophage
  4. phagemid

Answer : 3

Question 22 : λ ZAP vector is an example of

  1. phage
  2. phagemid
  3. cosmid
  4. plasmid

Answer : 2

Question 23 : λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

  1. 6-7 kb
  2. 1-2 kb
  3. 40-44 kb
  4. 20-23 kb

Answer : 1

Question 24 : Select the wrong statement about plasmids?

  1. It is extrachromosomal
  2. It is double stranded
  3. Its replication depends upon host cell
  4. It is closed and circular DNA

Answer : 3

Question 25 : Stuffer is

  1. the right arm of the vector DNA
  2. the left arm of the vector DNA
  3. central fragment of the vector DNA
  4. none of the above

Answer : 3

Question 26 : Conjugative plasmids

  1. exhibit antibiotic resistance
  2. do not exhibit antibiotic resistance
  3. carry transfer genes called the tra genes
  4. do not carry transfer genes

Answer : 3

Question 27 : Maximum size of foreign DNA that can be inserted into a replacement vector is

  1. 25-30 kb
  2. 18-20 kb
  3. 20-25 kb
  4. 40-50 kb

Answer : 3

Question 28 : Which of the following is not true about phagemid?

  1. Contain functional origin of replication of the plasmid and λ phage
  2. May be propagated as a plasmid or as phage in appropriate strain
  3. Contain λ att site
  4. Can only be propagated as phage

Answer : 4

Question 29 : pBR 322 has/have which of the following selection marker(s)?

  1. Ampr
  2. Tetr
  3. Both (1) and (2)
  4. Kanr

Answer : 3

Question 30 : A plasmid can be considered as a suitable cloning vector if

  1. it can be readily isolated from the cells
  2. it possesses a single restriction site for one or more restriction enzymes
  3. insertion of foreign DNA does not alter its replication properties
  4. All of the above

Answer : 4

Question 31 : Difference between λ gt 10 and λ gt 11 vectors is that

  1. λ gt 11 is an expression vector
  2. λ gt 10 is an expression vector
  3. λ gt 10 is a replacement vector
  4. λ gt 11 is a replacement vector

Answer : 1