Media Required for Plant Cell Culture Questions and Answers

Multiple Choice Questions and Answers on Media Required for Plant Cell Culture

Question 1 : Which of the following is not true about nurse or conditioned medium?

  1. It is liquid removed from the suspension of fast growing cells
  2. It contains uncharacterized growth factor released by growing cells
  3. It is used in the culture of regenerating protoplast
  4. it is removed aseptically from the culture and is autoclaved before use

Answer : 4

Question 2 : Very high sugar concentration (40-100 g/l) have been used

  1. in specialized secondary metabolite production
  2. to adjust the osmotic potential of the media in short term treatment for regeneration
  3. Both (a) and (b)
  4. none of these

Answer : 3

Question 3 : What is ‘nurse’ or conditioned medium?

  1. It is the media full of growth factors used for the growth of cells
  2. It is the medium added to nurse the callus culture
  3. Both (a) and (b)
  4. It is the liquid medium removed from the suspension of fast growing cells

Answer : 4

Question 4 : What are the macronutrients used in plant cell culture medium?

  1. N, P, K, S, Na
  2. N, P, K, Ca, Cl
  3. N, P, K, S, Ca
  4. N, P, Ca, Na, Cl

Answer : 3

Question 5 : Neutralized activated charcoal is occasionally added to young regenerating cultures to

  1. remove toxic phenolics produced by the stressed plant cell
  2. help to remove plants growth regulators introduced at an earlier stage
  3. both (1) and (2)
  4. maintain the pH of the medium

Answer : 3

Question 6 : Which is/are the naturally occurring plant auxins?

  1. Indole acetic acid (IAA)
  2. Naphthalenacetic acid (NAA)
  3. 2,4-dichlorophenoxyacetic acid
  4. All of the above

Answer : 1

Question 7 : Which is/are the disadvantage/(s) of using IAA in plant cell culture media?

  1. It is unstable in solution
  2. Gets easily oxidized
  3. Conjugated to inactive form by plant cells
  4. All of the above

Answer : 4

Question 8 : To maintain the pH of the culture

  1. organic acid such as citric, fumaric, malic and succinic acid is used
  2. synthetic buffers such as Tris, MES or HEPS are used
  3. both (1) and (2)
  4. ammonium salts are used

Answer : 3

Question 9 : Which of the following is not a cytokinin?

  1. 2,4-dichlorophenoxyacetic acid
  2. 6 benzylaminopurine
  3. Zeatin
  4. Kinetin

Answer : 1

Question 10 : Which of the following is not an auxin?

  1. Indole acetic acid (IAA)
  2. Naphthalenacetic acid (NAA)
  3. zeatin
  4. Indole butyric acid

Answer : 3

Question 11 : Which of the following growth regulator is used to stimulate embryo or shoot development?

  1. Auxins
  2. Cytokinins
  3. Gibberellins
  4. Brassinosteroids

Answer : 3

Question 12 : Which of the following growth regulator cause plant cells to grow?

  1. Auxins
  2. Cytokinins
  3. Gibberellins
  4. Brassinosteroids

Answer : 1

Question 13 : Silver thiosulphate is added to culture medium as it helps to

  1. maintain the pH
  2. remove toxic phenolics from plant cells
  3. prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
  4. all of the above

Answer : 3

Question 14 : In plant cell culture media, auxins and cytokinins are used in the range of

  1. 1-50μM
  2. 50-100μM
  3. 100-125μM
  4. more than 125μM

Answer : 1

Question 15 : Concentration of sucrose generally used in plant cell culture media is

  1. 10-15 g/l
  2. 20-30 g/l
  3. 40-50 g/l
  4. 60-70 g/l

Answer : 2

Question 16 : Which is the most common carbon source used in the plant cell culture media?

  1. Sucrose
  2. Glucose
  3. Fructose
  4. Maltose

Answer : 1

Question 17 : Which of the following is an ethylene biosynthesis inhibitor?

  1. Citric acid
  2. Succinic acid
  3. Activated charcoal
  4. Silver thiosulphate

Answer : 4

Question 18 : Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

  1. utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
  2. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
  3. utilization of both ammonium and nitrate result in rise in pH
  4. utilization of both ammonium and nitrate result in drop in pH

Answer : 1

Question 19 : Which of the following growth regulator is added for short initiation during plant regeneration from callus?

  1. Auxins
  2. Cytokinins
  3. Gibberellins
  4. Brassinosteroids

Answer : 2

Question 20 : Which of the following growth regulator promote cell division?

  1. Auxins
  2. Cytokinins
  3. Gibberellins
  4. Brassinosteroids

Answer : 2