Protein and Nucleic Acid Interactions Questions and Answers

Multiple Choice Questions and Answers on Protein and Nucleic Acid Interactions

Question 1 : Cleavage of DNA by EcoRI endonuclease results in the formation of

  1. two 3′-OH ends and two 5′-phosphate ends
  2. two 3′-OH ends and two 5′-phosphate ends
  3. two 3′-OH ends and two 5′-phosphate ends
  4. one 3′-OH end and two 5′-phosphate end

Answer : 1

Question 2 : Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

  1. Ile and Val
  2. Lys and Arg
  3. Leu and Ala
  4. Cys and Met

Answer : 2

Question 3 : Reverse transcripase from HIV does not

  1. uses RNA as a template
  2. has a 5′ to 3′ polymerase activity
  3. has a 3′- 5′ exonuclease activity
  4. requires a primer

Answer : 3

Question 4 : Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

  1. Asn and Gln
  2. Cys and Met
  3. Lys and Leu
  4. Ile and Val

Answer : 1

Question 5 : Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

  1. positive direction
  2. 3′ to 5′ direction
  3. 5′ to 3′ direction
  4. negative direction

Answer : 3

Question 6 : The type II restriction endonucleases, bind to DNA sites

  1. using H-bonds for specificity
  2. that are usually palindromic
  3. using non-sequence-dependent backbone interactions
  4. all of the above

Answer : 4

Question 7 : The enzyme that joins DNA cuts is called

  1. joinase
  2. ligase
  3. DNA phosphorylase
  4. reverse transcriptase

Answer : 2