Multiple Choice Questions and Answers on Protein and Nucleic Acid Interactions
Question 1 : Cleavage of DNA by EcoRI endonuclease results in the formation of
- two 3′-OH ends and two 5′-phosphate ends
- two 3′-OH ends and two 5′-phosphate ends
- two 3′-OH ends and two 5′-phosphate ends
- one 3′-OH end and two 5′-phosphate end
Answer : 1
Question 2 : Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
- Ile and Val
- Lys and Arg
- Leu and Ala
- Cys and Met
Answer : 2
Question 3 : Reverse transcripase from HIV does not
- uses RNA as a template
- has a 5′ to 3′ polymerase activity
- has a 3′- 5′ exonuclease activity
- requires a primer
Answer : 3
Question 4 : Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?
- Asn and Gln
- Cys and Met
- Lys and Leu
- Ile and Val
Answer : 1
Question 5 : Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
- positive direction
- 3′ to 5′ direction
- 5′ to 3′ direction
- negative direction
Answer : 3
Question 6 : The type II restriction endonucleases, bind to DNA sites
- using H-bonds for specificity
- that are usually palindromic
- using non-sequence-dependent backbone interactions
- all of the above
Answer : 4
Question 7 : The enzyme that joins DNA cuts is called
- joinase
- ligase
- DNA phosphorylase
- reverse transcriptase
Answer : 2