Recombinant DNA Technology
Multiple Choice Questions and Answers
1. Combining the DNA’s of two species into a single DNA molecule is called
(a) genetic recombination
(b) recombinant DNA techniques
(c) crossing over
(d) gene amplification
Answer: (b) recombinant DNA techniques
2. Which of the following is not a cloning vector?
(a) Agrobacterium
(b) Yeast
(c) Neurospora
(d) Bacteriophage
Answer: (c) Neurospora
3. DNA can be separated by
(a) gel electrophoresis
(b) autoradiography
(c) x-ray crystallography
(d) centrifugation
Answer: (a) gel electrophoresis
4. Genomic library can be prepared by
(a) PCR technique
(b) shotgun experiment
(c) colony hybridization
(d) all of these
Answer: (b) shotgun experiment
5. Plasmids are used in genetic engineering because they
(a) are easily available
(b) can replicate
(c) can integrate with hosts chromosome
(d) are inert
Answer: (c) can integrate with hosts chromosome
6. Which of the following is a commonly used bacteria in plant genetic engineering?
(a) Salmonella typhii
(b) Bacillus subtilis
(c) Corynebacterium
(d) Agrobacterium
Answer: (d) Agrobacterium
7. Monoclonal antibodies are produced by
(a) pure culture of cells producing antibodies
(b) recombinant DNA technology
(c) somatic hybridization technology
(d) DNA-RNA hybridization
Answer: (c) somatic hybridization technology
8. Genetically engineered bacteria is used for the production of
(a) thyroxine
(b) human insulin
(c) ephedrine
(d) cortisoles
Answer: (b) human insulin
9. Which of the following enzyme is used to join bits of DNA?
(a) ligase
(b) primase
(c) DNA polymerase
(d) endouclease
Answer: (a) ligase
10. The sites of DNA where restriction enzyme act are generally
(a) palindromic
(b) tandem repeats
(c) CG rich regions
(d) TATA boxes
Answer: (a) palindromic
11. Which of the following enzyme is necessary to make accurate copies of vector DNA?
(a) RNA polymerase
(b) DNA polymerase
(c) DNA ligase
(d) Endonucleases
Answer: (d) Endonucleases
12. What is the name of the site where foreign DNA can be inserted into in the plasmid of Agrobacterium?
(a) t-RNA
(b) c-DNA
(c) T-DNA
(d) B-DNA
Answer: (c) T-DNA
13. A method used to insert DNA molecules into the cells by using short electrical impulses is known as
(a) biolistics
(b) microinjection
(c) liposomes
(d) electroporation
Answer: (d) electroporation
14. Disease-causing organisms are identified by using
(a) MAb’s
(b) microprojectiles
(c) DNA probes
(d) gene transfer
Answer: (c) DNA probes
15. The most effective treatment for genetic disorder in the present times is
(a) gene mapping
(b) genetic counselling
(c) gene therapy
(d) cloning
Answer: (c) gene therapy
16. Genetically identical cells produced as a result of asexual reproduction are called
(a) clones
(b) siblings
(c) offsprings
(d) hybrids
Answer: (a) clones
17. Plant cells can easily generate into an entire plant because
(a) these are totipotent
(b) embryo is totipotent
(c) stem cells are pluripotent
(d) they divide actively
Answer: (a) these are totipotent
18. Transgenic organisms are produced by
(a) addition, deletion or modification of genes
(b) genetic transformation by bacteria
(c) chromosomal aberrations
(d) genetic mutations
Answer: (a) addition, deletion or modification of genes
19. Technique of DNA fingerprinting involves
(a) ELISA
(b) Northern blotting
(c) Southern blotting
(d) PAGE
Answer: (c) Southern blotting
20. Gene library is
(a) set of books on genes
(b) variety of genetic characters of an organism
(c) DNA sequences
(d) collection of independently isolated DNA of an organism
Answer: (d) collection of independently isolated DNA of an organism
21. Which of the following is used for the production of c DNA?
(a) DNA polymerase
(b) Reverse transcriptase
(c) Endonucleases
(d) Ligases
Answer: (b) Reverse transcriptase
22. Gene bank store
(a) genes only
(b) seeds only
(c) gene maps only
(d) germplasm
Answer: (d) germplasm
23. Cells obtained from cancerous tumors are called
(a) galls
(b) myelomas
(c) antibodies
(d) hybridomas
Answer: (b) myelomas
24. The enzymes commonly used in genetic engineering are
(a) restriction endonuclease and ligase
(b) ligase and polymerase
(c) restriction endonuclease and polymerases
(d) endonuclease and ligase
Answer: (a) restriction endonuclease and ligase
25. The first hormone produced artificially by culturing bacteria is
(a) insulin
(b) adrenaline
(c) thyroxine
(d) testosterone
Answer: (a) insulin
26. Antiviral substance produced by man, vertebrates response to viral infection for resisting multiplication of virus is known as
(a) virion
(b) interferon
(c) antigen
(d) antivirin
Answer: (b) interferon
27. If a person shows production of interferons in his body, the chances are that he has got an infection of
(a) thyroid
(b) measles
(c) tetanus
(d) malaria
Answer: (b) measles
28. Ti plasmid in transforms cells of
(a) animals
(b) plants
(c) bacteria
(d) fungi
Answer: (b) plants
29. DNA is genetically methylated at
(a) A-base
(b) G-base
(c) T-base
(d) C- base
Answer: (d) C- base
30. Bacteria protect themselves from viruses by fragmenting viral DNA with
(a) endonuclease
(b) exonuclease
(c) gyrase
(d) ligase
Answer: (a) endonuclease
31. The enzyme used in PCR technology is
(a) RNA polymerase
(b) Taq polymerase
(c) reverse transcriptase
(d) both b and c
Answer: (b) Taq polymerase
32. What is true for plasmids?
(a) found in viruses
(b) contain genes for vital activities
(c) part of nuclear chromosomes
(d) widely used in gene transfer
Answer: (d) widely used in gene transfer
33. Transgenic plants are plants having
(a) no gene
(b) genes in transposition
(c) genes have no function
(d) genes of other organism
Answer: (d) genes of other organism
34. Construction of recombinant DNA involves
(a) cleaving and joining of DNA segments with endonuclease
(b) cleaving DNA segments with endonuclease and rejoining with ligase
(c) cleaving and rejoining DNA segments with ligase
(d) cleaving DNA segments with ligase and rejoining with endonuclease
Answer: (b) cleaving DNA segments with endonuclease and rejoining with ligase
35. Natural genetic engineer is
(a) Pseudomonas putida
(b) Agrobacterium tumefaciens
(c) Escherichia coli
(d) Bacillus subtilis
Answer: (b) Agrobacterium tumefaciens
36. Introduction of genetically modified food is not desirable because
(a) it will affect economy of developing countries
(b) the products are less testly
(c) they are costly
(d) there is danger of entry of toxins and viruses in food
Answer: (d) there is danger of entry of toxins and viruses in food
37. The first transgenic crop was
(a) pea
(b) flax
(c) tobacco
(d) cotton
Answer: (c) tobacco
38. Producing a ‘giant mouse’ in the laboratory was possible through
(a) gene mutation
(b) gene manipulation
(c) gene synthesis
(d) gene duplication
Answer: (b) gene manipulation
39. The new strain of bacteria produce by biotechnology in alcohol industry is
(a) Escherichia coli
(b) Saccharomyces cerevisiae
(c) Bacillus subtilis
(d) Pseudomonas putida
Answer: (d) Pseudomonas putida
40. Antiviral substance produced by man, vertebrates in response to viral infection for resisting multiplication of virus is known as
(a) virion
(b) interferon
(c) antigen
(d) antivirus
Answer: (b) interferon
41. Which of the following is a transgenic animal having a human gene for production of a specific protein?
(a) Daily
(b) Charlie and George
(c) Megon
(d) Tetra
Answer: (b) Charlie and George
42. The basic method of producing transgenic animals are
(a) DNA microinjection
(b) retrovirus mediated gene transfer
(c) embryonic stem cell mediated gene transfer
(d) all of these
Answer: (d) all of these
43. Which of the following recombinant protein is used to dissolve blood clots causing acute myocardial infaction?
(a) insulin
(b) interferons
(c) tissue plaminogen activator
(d) anti haemophilic factor
Answer: (c) tissue plaminogen activator
44. Insect tolerant gene from from Bacillus thuringiensis is introduced using plasmid of
(a) Escherichia coli
(b) Haemophilus influenza
(c) Agrobacterium tumefaciens
(d) Arabidopsis thaliana
Answer: (c) Agrobacterium tumefaciens
45. pBR322 is a plasmid belonging to
(a) yeat
(b) all bacteria
(c) Escherichia coli
(d) bacteriophages
Answer: (c) Escherichia coli
46. Bt toxin becomes active when in the insect gut because
(a) the conditions are acidic
(b) it is tissue specific
(c) of favourable alkaline pH
(d) none of these
Answer: (c) of favourable alkaline pH
47. Restriction endonucleases are utilised in genetic engineering as
(a) molecular build up at nucleotides
(b) molecular degradation to DNA break up
(c) molecular scalpels for cutting DNA bits into long chains
(d) molecular cement for combining DNA bits into long chains
Answer: (c) molecular scalpels for cutting DNA bits into long chains
48. DNA fingerprinting refers to
(a) molecular analysis of profiles of DNA samples
(b) Analysis of samples using imprinting devices
(c) technique used for molecular analysis of different specimens of DNA
(d) techniques used for identification of fingerprints of individuals
Answer: (a) molecular analysis of profiles of DNA samples
49. Restriction endonucleases
(a) are present in mammalian cell for degradation of DNA when the cell dies
(b) are used in genetic engineering for ligating two DNA molecules
(c) are used for in vitro DNA synthesis
(d) are synthesized by bacteria as part of their defence mechanism
Answer: (d) are synthesized by bacteria as part of their defence mechanism
50. Restriction enzymes are used in genetic engineering because they
(a) can cut DNA at specific base sequence
(b) are proteolytic enzymes which can degrade harmful proteins
(c) are nucleases that cut DNA at variable sites
(d) can join different DNA fragments
Answer: (a) can cut DNA at specific base sequence