Recombinant DNA Technology (Multiple Choice Questions and Answers)

Recombinant DNA Technology

Multiple Choice Questions and Answers

1. Combining the DNA’s of two species into a single DNA molecule is called

(a) genetic recombination

(b) recombinant DNA techniques

(c) crossing over

(d) gene amplification

Answer: (b) recombinant DNA techniques

2. Which of the following is not a cloning vector?

(a) Agrobacterium

(b) Yeast

(c) Neurospora

(d) Bacteriophage

Answer: (c) Neurospora

3. DNA can be separated by

(a) gel electrophoresis

(b) autoradiography

(c) x-ray crystallography

(d) centrifugation

Answer: (a) gel electrophoresis

4. Genomic library can be prepared by

(a) PCR technique

(b) shotgun experiment

(c) colony hybridization

(d) all of these

Answer: (b) shotgun experiment

5. Plasmids are used in genetic engineering because they

(a) are easily available

(b) can replicate

(c) can integrate with hosts chromosome

(d) are inert

Answer: (c) can integrate with hosts chromosome

6. Which of the following is a commonly used bacteria in plant genetic engineering?

(a) Salmonella typhii

(b) Bacillus subtilis

(c) Corynebacterium

(d) Agrobacterium

Answer: (d) Agrobacterium

7. Monoclonal antibodies are produced by

(a) pure culture of cells producing antibodies

(b) recombinant DNA technology

(c) somatic hybridization technology

(d) DNA-RNA hybridization

Answer: (c) somatic hybridization technology

8. Genetically engineered bacteria is used for the production of

(a) thyroxine

(b) human insulin

(c) ephedrine

(d) cortisoles

Answer: (b) human insulin

9. Which of the following enzyme is used to join bits of DNA?

(a) ligase

(b) primase

(c) DNA polymerase

(d) endouclease

Answer: (a) ligase

10. The sites of DNA where restriction enzyme act are generally

(a) palindromic

(b) tandem repeats

(c) CG rich regions

(d) TATA boxes

Answer: (a) palindromic

11. Which of the following enzyme is necessary to make accurate copies of vector DNA?

(a) RNA polymerase

(b) DNA polymerase

(c) DNA ligase

(d) Endonucleases

Answer: (d) Endonucleases

12. What is the name of the site where foreign DNA can be inserted into in the plasmid of Agrobacterium?

(a) t-RNA

(b) c-DNA

(c) T-DNA

(d) B-DNA

Answer: (c) T-DNA

13. A method used to insert DNA molecules into the cells by using short electrical impulses is known as

(a) biolistics

(b) microinjection

(c) liposomes

(d) electroporation

Answer: (d) electroporation

14. Disease-causing organisms are identified by using

(a) MAb’s

(b) microprojectiles

(c) DNA probes

(d) gene transfer

Answer: (c) DNA probes

15. The most effective treatment for genetic disorder in the present times is

(a) gene mapping

(b) genetic counselling

(c) gene therapy

(d) cloning

Answer: (c) gene therapy

16. Genetically identical cells produced as a result of asexual reproduction are called

(a) clones

(b) siblings

(c) offsprings

(d) hybrids

Answer: (a) clones

17. Plant cells can easily generate into an entire plant because

(a) these are totipotent

(b) embryo is totipotent

(c) stem cells are pluripotent

(d) they divide actively

Answer: (a) these are totipotent

18. Transgenic organisms are produced by

(a) addition, deletion or modification of genes

(b) genetic transformation by bacteria

(c) chromosomal aberrations

(d) genetic mutations

Answer: (a) addition, deletion or modification of genes

 19. Technique of DNA fingerprinting involves

(a) ELISA

(b) Northern blotting

(c) Southern blotting

(d) PAGE

Answer: (c) Southern blotting

20. Gene library is

(a) set of books on genes

(b) variety of genetic characters of an organism

(c) DNA sequences

(d) collection of independently isolated DNA of an organism

Answer: (d) collection of independently isolated DNA of an organism

 21. Which of the following is used for the production of c DNA?

(a) DNA polymerase

(b) Reverse transcriptase

(c) Endonucleases

(d) Ligases

Answer: (b) Reverse transcriptase

 22. Gene bank store

(a) genes only

(b) seeds only

(c) gene maps only

(d) germplasm

Answer: (d) germplasm

 23. Cells obtained from cancerous tumors are called

(a) galls

(b) myelomas

(c) antibodies

(d) hybridomas

Answer: (b) myelomas

 24. The enzymes commonly used in genetic engineering are

(a) restriction endonuclease and ligase

(b) ligase and polymerase

(c) restriction endonuclease and polymerases

(d) endonuclease and ligase

Answer: (a) restriction endonuclease and ligase

 25. The first hormone produced artificially by culturing bacteria is

(a) insulin

(b) adrenaline

(c) thyroxine

(d) testosterone

Answer: (a) insulin

 26. Antiviral substance produced by man, vertebrates response to viral infection for resisting multiplication of virus is known as

(a) virion

(b) interferon

(c) antigen

(d) antivirin

Answer: (b) interferon

27. If a person shows production of interferons in his body, the chances are that he has got an infection of

(a) thyroid

(b) measles

(c) tetanus

(d) malaria

Answer: (b) measles

 28. Ti plasmid in transforms cells of

(a) animals

(b) plants

(c) bacteria

(d) fungi

Answer: (b) plants

 29. DNA is genetically methylated at

(a) A-base

(b) G-base

(c) T-base

(d) C- base

Answer: (d) C- base

 30. Bacteria protect themselves from viruses by fragmenting viral DNA with

(a) endonuclease

(b) exonuclease

(c) gyrase

(d) ligase

Answer: (a) endonuclease

 31. The enzyme used in PCR technology is

(a) RNA polymerase

(b) Taq polymerase

(c) reverse transcriptase

(d) both b and c

Answer: (b) Taq polymerase

 32. What is true for plasmids?

(a) found in viruses

(b) contain genes for vital activities

(c) part of nuclear chromosomes

(d) widely used in gene transfer

Answer: (d) widely used in gene transfer

 33. Transgenic plants are plants having

(a) no gene

(b) genes in transposition

(c) genes have no function

(d) genes of other organism

Answer: (d) genes of other organism

 34. Construction of recombinant DNA involves

(a) cleaving and joining of DNA segments with endonuclease

(b) cleaving DNA segments with endonuclease and rejoining with ligase

(c) cleaving and rejoining DNA segments with ligase

(d) cleaving DNA segments with ligase and rejoining with endonuclease

Answer: (b) cleaving DNA segments with endonuclease and rejoining with ligase

35. Natural genetic engineer is

(a) Pseudomonas putida

(b) Agrobacterium tumefaciens

(c) Escherichia coli

(d) Bacillus subtilis

Answer: (b) Agrobacterium tumefaciens

 36. Introduction of genetically modified food is not desirable because

(a) it will affect economy of developing countries

(b) the products are less testly

(c) they are costly

(d) there is danger of entry of toxins and viruses in food

Answer: (d) there is danger of entry of toxins and viruses in food

37. The first transgenic crop was

(a) pea

(b) flax

(c) tobacco

(d) cotton

Answer: (c) tobacco

38. Producing a ‘giant mouse’ in the laboratory was possible through

(a) gene mutation

(b) gene manipulation

(c) gene synthesis

(d) gene duplication

Answer: (b) gene manipulation

39. The new strain of bacteria produce by biotechnology in alcohol industry is

(a) Escherichia coli

(b) Saccharomyces cerevisiae

(c) Bacillus subtilis

(d) Pseudomonas putida

Answer: (d) Pseudomonas putida

40. Antiviral substance produced by man, vertebrates in response to viral infection for resisting multiplication of virus is known as

(a) virion

(b) interferon

(c) antigen

(d) antivirus

Answer: (b) interferon

41. Which of the following is a transgenic animal having a human gene for production of a specific protein?

(a) Daily

(b) Charlie and George

(c) Megon

(d) Tetra

Answer: (b) Charlie and George

42. The basic method of producing transgenic animals are

(a) DNA microinjection

(b) retrovirus mediated gene transfer

(c) embryonic stem cell mediated gene transfer

(d) all of these

Answer: (d) all of these

43. Which of the following recombinant protein is used to dissolve blood clots causing acute myocardial infaction?

(a) insulin

(b) interferons

(c) tissue plaminogen activator

(d) anti haemophilic factor

Answer: (c) tissue plaminogen activator

 44. Insect tolerant gene from from Bacillus thuringiensis is introduced using plasmid of

(a) Escherichia coli

(b) Haemophilus influenza

(c) Agrobacterium tumefaciens

(d) Arabidopsis thaliana

Answer: (c) Agrobacterium tumefaciens

45. pBR322 is a plasmid belonging to

(a) yeat

(b) all bacteria

(c) Escherichia coli

(d) bacteriophages

Answer: (c) Escherichia coli

 46. Bt toxin becomes active when in the insect gut because

(a) the conditions are acidic

(b) it is tissue specific

(c) of favourable alkaline pH

(d) none of these

Answer: (c) of favourable alkaline pH

 47. Restriction endonucleases are utilised in genetic engineering as

(a) molecular build up at nucleotides

(b) molecular degradation to DNA break up

(c) molecular scalpels for cutting DNA bits into long chains

(d) molecular cement for combining DNA bits into long chains

Answer: (c) molecular scalpels for cutting DNA bits into long chains

 48. DNA fingerprinting refers to

(a) molecular analysis of profiles of DNA samples

(b) Analysis of samples using imprinting devices

(c) technique used for molecular analysis of different specimens of DNA

(d) techniques used for identification of fingerprints of individuals

Answer: (a) molecular analysis of profiles of DNA samples

 49. Restriction endonucleases

(a) are present in mammalian cell for degradation of DNA when the cell dies

(b) are used in genetic engineering for ligating two DNA molecules

(c) are used for in vitro DNA synthesis

(d) are synthesized by bacteria as part of their defence mechanism

Answer: (d) are synthesized by bacteria as part of their defence mechanism

50. Restriction enzymes are used in genetic engineering because they

(a) can cut DNA at specific base sequence

(b) are proteolytic enzymes which can degrade harmful proteins

(c) are nucleases that cut DNA at variable sites

(d) can join different DNA fragments

Answer: (a) can cut DNA at specific base sequence