Multiple Choice Questions and Answers on Synthesis of Therapeutic Agents
Question 1 : The two chains of insulin can be separated by detaching it from β-galactosidase using
- hydrogen chloride
- cyanogen bromide
- peptidase
- protease
Answer : 2
Question 2 : The two chains are joined together to constitute native insulin using
- sodium dissulphonate and sodium sulphite
- sodium dissulphonate only
- sodium sulphate and sodium sulphite
- sodium sulphate only
Answer : 1
Question 3 : Foot and mouth disease (FMD) of animals is caused by
- RNA virus
- DNA virus
- bacteria
- protozoa
Answer : 1
Question 4 : In India first genetically engineered vaccine against HBV was developed by
- Ranbaxy Pvt Ltd
- Shantha Biotechnics Pvt Ltd
- Dabur Pvt Ltd
- Glaxo Pvt Ltd
Answer : 2
Question 5 : The recombinant hGH lacks
- terminal glutamine
- terminal lysine
- terminal methionine
- terminal arginine
Answer : 3
Question 6 : How is human insulin synthesized using recombinant DNA technology?
- By using chemically synthesized DNA sequences for the two chains separately
- By isolating DNA from the islets of Langerhans of pancreas
- By using cDNA for insulin
- By using chemically synthesized DNA sequences for the complete insulin protein
Answer : 1
Question 7 : Human growth hormone (hGH) is secreted by
- pitutary gland
- hypothalamus
- pancreas
- none of these
Answer : 1
Question 8 : The promoter used for cloning HBV surface antigen is
- galactokinase
- alcohol dehydrogenase 1
- β-galactosidase
- polyhedrin
Answer : 2
Question 9 : Recombinant vaccine for HBV was produced by cloning viral genes in
- plasmids
- cosmids
- autonomously replicating plasmid of yeast
- phagemids
Answer : 3
Question 10 : The first therapeutic product formed by means of recombinant DNA technology is
- human growth hormone
- insulin
- hepatitis B vaccine
- vaccine for foot and mouth disease
Answer : 2
Question 11 : Which one of the following capsid protein of picorna virus is immunogenic in nature?
- VP1
- VP2
- VP3
- VP4
Answer : 1
Question 12 : Human insulin formed by recombinant DNA technology is known as
- H insulin
- R insulin
- humulin
- huinsulin
Answer : 3
Question 13 : The gene coding for VP1 is cloned in
- pMB 9
- pBR 322
- pUC 18
- pUC 19
Answer : 2
Question 14 : How many nucleotides are there in Ss RNA molecule of picorna virus causing FMD?
- 1000
- 5000
- 5000
- 10000
Answer : 3
Question 15 : Recombinant vaccine for Hepatitis B virus has been synthesized against which of the following antigen?
- Viral core antigen (HBcAg)
- Viral surface antigen (HBsAg)
- e antigen (HBeAg)
- All of the above
Answer : 2
Question 16 : Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid pBR 322 by the side of
- β-Galactosidase
- galactokinase
- acid phosphatase
- glucokinase
Answer : 1