Multiple Choice Questions and Answers on Transgenic Plant Questions and Answers
Question 1 : Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of
- gene for ALS (acetolactate synthase)
- gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
- gene for GS (glutamine synthase)
- any of the above
Answer : 1
Question 2 : In transgenic plants that have been manipulated to express patho-genesis-related (PR) proteins all the time it has been shown that
- these provide no protection at all against pathogens
- expression of one PR protein provides high levels of protection against many pathogens
- expression of two PR proteins provides some protection against a few pathogens
- these proteins trigger the production of other antimicrobial defenses
Answer : 4
Question 3 : Which of the following gene detoxify herbicide bronoxynil?
- Nitrilase
- Glutathione S-transferase (GST)
- Phosphinothricin acetyl transferase
- All of these
Answer : 1
Question 4 : For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used?
- Target molecule is made insensitive to herbicide
- Target protein is overproduced
- A pathway should be introduced that detoxify the herbicide
- All of the above
Answer : 4
Question 5 : Which of the following is not a characteristic of a transgenic crop?
- herbicide resistance
- Bt insect resistance toxin
- increased methionine content
- none of these
Answer : 4
Question 6 : Phosphinothricin acetyl transferase is encoded by
- gene bxn in Klebsiella pneumoniae
- bar gene in Streptomyces spp
- both (1) and (2)
- none of these
Answer : 2
Question 7 : Reducing the expression of pectin methylesterase (PME) has resulted in
- plants with resistance to bacteria and fungi
- tomato juice that has higher viscosity
- tomato fruit that last longer before they become rotten
- tomato fruit that will not ripen
Answer : 2
Question 8 : Resistance to glyphosphate in transgenic petunia plants has been developed by the transfer of
- gene for EPSPS(5-enol-pyruvyl shikimat 3 phosphate synthase)
- gene for ALS (acetolactate synthase)
- gene for GS (glutamine synthase)
- any of the above
Answer : 1
Question 9 : Which of the following compounds has been produced in transgenic plants to improve tolerance to salt stress and water deficit?
- Sucrose
- Mannitol
- Nicotine
- Octopine
Answer : 2
Question 10 : Expression of antisense RNA in transgenic plants is a general method used to
- activate the expression of all genes in a biochemical pathway
- eliminate the expression of all genes in a biochemical pathway
- block the expression of virus coat protein genes
- reduce or eliminate the expression of individual genes
Answer : 4
Question 11 : Tomatoes exhibiting delayed ripening express antisense RNA against
- glycerol 1 phosphate acyl transferase
- polygalactouranase
- ACC deaminase
- sucrose phosphate synthase gene
Answer : 3
Question 12 : Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?
- AUUAAGGC
- UAAUUCCG
- CGGAATTA
- TAATTCCG
Answer : 1
Question 13 : Resistance to L-phosphinothrium in tobacco plants is developed by the transfer of
- gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase)
- gene for ALS (acetolactate synthase)
- gene for GS (glutamine synthase)
- any of the above
Answer : 3
Question 14 : A __________ is a small laboratory set up to simulate conditions of a particular environment.
- microcosm
- mibridization
- bioremediation
- rhizosecretion
Answer : 1
Question 15 : The roots of a plant are converted into drug-producing structures in a process called
- microcosm establishment
- mibridization
- bioremediation
- rhizosecretion
Answer : 4
Question 16 : ACC demainase gene is responsible for
- synthesis for ethylene
- degradation of ACC an immediate precursor to ethylene
- both (1) and (2)
- synthesis of polygalactouranase
Answer : 2
Question 17 : Two genes from a bacterium (Alcaligenes eutrophus) and a cotton gene produce
- a plastic
- a vaccine against the bacterium
- a pharmaceutical product
- herbicide resistance
Answer : 1
Question 18 : The first field tests were conducted with which of the following genetically altered organism?
- bt Corn
- Vaccinia virus containing a gene from the rabies virus
- The flavrsavr tomato
- Strawberry seedlings sprayed with ice-minus bacteria
Answer : 4
Question 19 : The delayed ripening tomato was created by a biotechnologist who __________ a gene.
- altered
- silenced
- replaced
- relocated
Answer : 2
Question 20 : Antisense technology
- selectively blocks expression of a gene
- combines genetic material from different species
- combines organelles and cells
- alters or transfers cells
Answer : 1
Question 21 : Antisense transgenic plants produced fruit that softened
- more slowly than the normal fruit
- more rapidly than the normal fruit
- as the normal fruits
- none of these
Answer : 1
Question 22 : Insect resistance in the transgenic plant has been achieved by
- transferring genes for Bt toxins
- transferring genes for protease inhibitors
- transferring genes for other insecticidal secondary metabolities
- all of the above
Answer : 4
Question 23 : Which of the following genes can be used for making resistances against viral infection?
- Genes for capsid protein
- Gene for nucleocapsid protein
- Satellite RNA
- All of these
Answer : 4
Question 24 : Which of the following has been widely used to provide resistance against plant viruses?
- Virus resistance genes from bacteria
- Expression of virus coat protein genes in transgenic plants
- Expression of anti-virus genes in vectors that transmit viruses
- Expression of ribonuclease (RNase) genes in host plants
Answer : 2
Question 25 : Cross protection against viruses in transgenic plants can be obtained by
- inoculating the susceptible strain of a crop with a mild strain of a virus that helps in developing resistance against more virulent strain
- inoculating the susceptible strain with the coat proteins of virulent strain
- inoculating the susceptible strain with genes of nucleocapsid
- any of the above
Answer : 1
Question 26 : Bruise resistant tomatoes have been developed by the expression of antisense RNA against
- glycerol 1 phosphate acyl transferase
- polygalactouranase
- ACC deaminase
- Sucrose phosphate synthase gene
Answer : 2
Question 27 : Which cell-based technology endows a cell with increased ability to harness energy?
- Chlybridization
- Cybridization
- Mibridization
- Protoplast fusion
Answer : 1
Question 28 : Which of the following gene is transferred to plants that detoxify the herbicide atrazine?
- Nitrilase
- Glutathione S-transferase (GST)
- Phosphinothrium acetyl transferase
- All of these
Answer : 2
Question 29 : Which of the following self-pollinating plant/(s) tend to be homozygous?
- Peas
- Tomato
- Peach
- All of these
Answer : 4
Question 30 : Which cell-based plant technology involves the combining of two cells without cell walls from different species?
- Clonal propagation
- Cybridization
- Mutant selection
- Protoplast fusion
Answer : 4
Question 31 : A naturally occurring variant, possessing characteristics of interest, is identified. This plant is selectively bred. This is an example of
- traditional plant breeding
- transgenic technology
- mutant selection
- none of these
Answer : 1
Question 32 : Which of the following dies from Ti plasmid infection?
- Rice
- Corn
- Sorghum
- All of these
Answer : 4
Question 33 : Transgenic plants
- contain foreign genes in their cells
- are used to produce human antibodies
- both (1) and (2)
- are plants that differ in geographical locations
Answer : 3
Question 34 : Low temperatures induce the expression of many cold-induced genes. Transgenic plants with improved cold tolerance have been produced by
- expressing the protein that activates expression of these genes all the time in plants
- cooling plants using the Peltier effect
- expressing a gene for production of antifreeze (ethylene glycol) in plants
- increasing evaporative cooling from leaf surfaces
Answer : 1
Question 35 : Nitrilase is encoded by
- gene bxn in Klebsiella pneumoniae
- bar gene in Streptomyces spp
- both (1) and (2)
- none of these
Answer : 1
Question 36 : If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective?
- Clonal propagation
- Cybridization
- Protoplast fusion
- Mutant selection
Answer : 4
Question 37 : What are the various disadvantages of cross protection?
- Possibility of mutations in inducing mild virus strain
- Possibility of synergism between inducing virus and other unrelated virus
- Possibility of unnecessary spread of mild virus
- All of the above
Answer : 4
Question 38 : Plants derived sexually from the same plant are____ while those derived from somatic tissue from the same plant are____
- identical, different
- different, also different
- different, identical
- plants cannot be derived from somatic tissue
Answer : 3
Question 39 : Transgenic plants with increased tolerance to aluminum have been produced by making plants that
- secrete phytosiderophores into the soil
- make more metal-binding peptides like phytochelatins
- bind aluminum to the cell wall
- secrete citrate into the soil
Answer : 4
Question 40 : Transplastomics
- targets genes in the chloroplast
- provides exceptionally low yields of protein products
- produces genes that are released in pollen
- offers little opportunity for practical use
Answer : 1
Question 41 : Plants containing genes encoding cytokines and blood clotting factors are used in
- nutrition improvement
- pharmaceutical production
- vaccine production
- textile production
Answer : 2
Question 42 : The first transgenic plants expressing engineered foreign genes were tobacco plants produced by the use of
- Agrobacterium tumefaciens
- Bacillus thuringiensis
- Arabidopsis thaliana
- Streptomyces hygroscopicus
Answer : 1
Question 43 : Which of the following metabolites are implicated in stress tolerance?
- Proline
- Betaines
- Both (1) and (2)
- Citrate
Answer : 3
Question 44 : Which of the agricultural challenges below cannot be solved with transgenic techniques?
- Crops are damaged by frost
- Crops are killed by a virus
- Public concern about safety of synthetic pesticides
- Public preference for organic vegetables
Answer : 4
Question 45 : Which of the following statements is true regarding genomics?
- Plant genomics lags behind similar efforts in animals and microorganisms
- Researchers can make use of genomic information even if the entire genome of an organism is not known
- Researchers are busy in trying to determine the genome of the potato plant
- All of the above
Answer : 4
Question 46 : Which of the following gene is responsible for resistance against chilling?
- Glycerol 1 phosphate acyl transferase
- Polygalactouranase
- ACC deaminase
- Sucrose phosphate synthase gene
Answer : 1
Question 47 : Which of the following gene detoxifies herbicide phosphinothricin?
- Nitrilase
- Glutathione S-transferase (GST)
- Phosphinothricin acetyl transferase
- Phosphinothricin acetyl transferase
Answer : 3
Question 48 : In a __________ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area.
- microcosm establishment
- mibridization
- bioremediation
- rhizosecretion
Answer : 3
Question 49 : The controversy regarding the use of Bt corn is that it
- is potentially harmful to monarch butterflies
- is a potential allergen to humans
- both (1) and (2)
- can contaminate groundwater
Answer : 3
Question 50 : Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?
- ACC (1-aminocyclopropane-1-carboxylic acid)
- SAM (S-adenosylmethionine)
- Both (1) and (2)
- AOA (aminooxyacetic acid)
Answer : 3
Question 51 : Starch content of potatoes can be increased by using a bacterial gene, known as
- sucrose phosphate synthase gene
- ADP glucose pyrophosphorylase gene
- polygalactouranase gene
- none of the above
Answer :2
Question 52 : Which tropical fruit crop has been successfully engineered to be protected against a lethal virus?
- Passion fruit
- Papaya
- Mango
- Lychee
Answer :2