What do you understand by oxidative phosphorylation? Describe the process of anaerobic respiration.


Oxidative phosphorylation is the synthesis of energy-rich ATP molecules with the help of energy liberated during oxidation of reduced coenzymes (NADH2 and FADH2), produced in respiration.  The enzyme required for this synthesis is called ATP synthase.  It is considered to be the 5th complex of electron transport chain.  ATP synthase is located in F1 or headpiece of F0– F1 or elementary particles.  The particles are present in the inner mitochondrial membrane.  ATP synthase becomes active in ATP formation only when there is a proton gradient having higher concentration of H+ or protons on the F0 side as compared to F1 side.  Increased proton concentration is produced in the outer chamber or outer surface of inner mitochondrial membrane by the pushing of protons with the help of energy liberated by passage of electrons from one carrier to another.  Transport of electrons from NADH2 over electron transport chain helps in pushing 3 pairs of proton to the outer chamber while 2 pairs of protons are sent outwardly during electron flow from FADH2 (as FADH2 donates its electron further down to the electron transport chain).

Higher proton concentration in the outer chamber causes the protons to pass inwardly into matrix or inner chamber through the inner membrane.  The inner membrane possesses special proton channels in the region of F0 (base) of the F0- F1 particles.  The flow of protons through the F0 channel induces F1 particle to function as ATP synthase.  The energy of proton gradient is used in attaching a phosphate radical to ATP by high-energy bond.  This produces ATP.  Oxidation of one molecule of NADH2 produces 3 ATP molecules while a similar oxidation of FADH2 forms 2 ATP molecules.

Two ATP molecules are produced during glycolysis and 2 ATP molecules are produced during double Krebs cycle.  Glycolysis also produces 2 molecules of NADH2.  Its reducing power is transferred to mitochondria for ATP synthesis.  For this, a shuttle system operates at the inner mitochondrial membrane.

Shuttle System

In aerobic respiration, complete oxidation of one glucose molecule produces 38 ATP molecules.  But the number of ATP molecules so produced may vary depending upon the mode of entry of NADH2 in mitochondria.   The two molecules of NADH2 formed in glycolysis cannot directly enter the membranes of mitochondria.  These enter into the mitochondria through a shuttle or indirect pathway.  Two such known shuttles area as follows:

  1. In this shuttle, electrons of NADH2 are accepted by malate. Each molecule of NADH2 yields 3 ATP molecules and as such, complete oxidation of one molecule of glucose would yield 38 ATP molecules.  This shuttle occurs in cell of heart, liver etc.


  1. In the second shuttle, electrons of NADH2 are accepted by FAD. Reduced FAD (i.e., FADH2) yields only 2 ATP molecules through ETS.  As such, in this shuttle only 36 ATP molecules are produced.  Such shuttle occurs in most of the eukaryotic cell.


The shuttle continuously transfers electron from cytoplasm to mitochondria and does not allow the accumulation of hydrogen molecules in the cytoplasm.

Oxidative phosphorylation 


It is a process of release of energy in enzymatically controlled incomplete degradation of organic food without oxygen being used as oxidant.  Anaerobic respiration is exclusive mode of respiration in some parasitic worms and microorganism for example (bacteria, moulds).  Anaerobic respiration occurs in the roots of some waterlogged plants, muscles of animals, and as a supplementary mode of respiration in massive tissues. Fermentation is a kind of anaerobic respiration carried out primarily by fungi and bacteria Louis Pasteur (1822-1895) concluded that fermentation occurred only when living yeast cells were present.  However, Buchner (1897) found that living yeast cells were not necessary for fermentation.  He demonstrated that extract prepared by crushing yeast cells could ferment sugar.  This extract has been shown to contain an enzyme, zymase capable of fermenting hexose sugar.  Besides zymase, yeast cell also contains enzymes like sucrase and maltase, which can ferment sucrose and maltose respectively.  Direct fermentation of starch by yeast is not possible as it lacks amylase enzyme. Because of the enzyme zymase, fermentation is also called zymosis.

Commonly, fermentation is defined as the anaerobic breakdown of carbohydrates and other organic compounds into alcohols, organic acids, gases etc., with the help of microorganisms or their enzymes.  The mechanism of anaerobic respiration is similar to common pathway of aerobic respiration up to glycolysis.  Glycolysis breaks down glucose enzymatically in several steps to form two molecules of pyruvate.  Pyruvate is anaerobically broken down to yield various products depending upon the organism and the types of tissue.  The two common products are ethyl alcohol and lactic acid.


  1. Ethyl alcohol fermentation: It is quite common in fungi (e.g., Rhizopus, yeast) and bacteria.  Yeast can respire both aerobically and anaerobically.  Anaerobic respiration occurs in sugary solution if the fungus is not in contact with the atmosphere.  It causes fermentation.  In the presence of enzyme pyruvate decarboxylase and TPP (thiamine pyrophosphate), pyruvate is broken down to form acetaldehyde releasing carbon dioxide.


Pyruvate→Acetaldehyde+carbon dioxide


Acetaldehyde is reduced to ethyl alcohol or ethanol in the presence of enzyme alcohol dehydrogenase.  Hydrogen is obtained from NADH2 produced during oxidation of glyceraldehyde 3 phosphate to 1, 3 diphosphoglycerate in glycolysis.







  1. Lactic acid fermentation: The fermentation occurs in lactic acid bacteria (e.g., Lactobacillus), some fungi and muscles.  Lactic acid produced in muscles is sent to liver to regenerate glucose.  In lactic acid fermentation, pyruvate produced in glycolysis is directly reduced by NADH2 to form lactic acid.  No carbon dioxide is produced.  The enzyme is lactic dehydrogenase, which requires FMN (Flavin mononucleotide) and Zn2+.


Pyruvic acid+NADH2            →lactic acid+NAD+


Anaerobic respiration produces very little energy as compared to aerobic respiration.  The reasons are:

  1. There is incomplete breakdown of respiratory substrate.
  2. At least one of the products of anaerobic respiration is organic, which can be further oxidised to release energy.
  3. NADH2 produced during glycolysis is often re-utilized.
  4. ATP formation does not occur during regeneration of NAD+.
  5. Oxygen is not used for receiving electrons and protons.

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