Vectors Use for Animal Cell Culture Questions and Answers

Multiple Choice Questions and Answers on Vectors Use for Animal Cell Culture

Question 1 : 5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

  1. His 3
  2. Lys 2
  3. Ura 3
  4. Trp 1

Answer : 3

Question 2 : Which of the following promoters is repressed in presence of glucose?

  1. Galactokinase (GAL 1)
  2. Acid phosphatase (PHOS)
  3. Both (1) and (2)
  4. Phosphoglycerate kinase (PGK)

Answer : 1

Question 3 : Which of the following can be used for reducing the size of gene inserts containing introns?

  1. Vaccinia virus
  2. Baculo virus
  3. Retro virus
  4. none of these

Answer : 3

Question 4 : Bovine papilloma virus has a circular double stranded DNA genome of about

  1. 2.5 kb
  2. 5.2 kb
  3. 7.9 kb
  4. 9.5 kb

Answer : 3

Question 5 : Retroviruses consist of

  1. Ds DNA genome
  2. Ss DNA genome
  3. RNA genome
  4. cDNA genome

Answer : 3

Question 6 : YAC vectors can be used to obtain artificial chromosomes because it carries

  1. centromere
  2. telomere
  3. autonomously replicating sequence
  4. all of these

Answer : 4

Question 7 : Maximum insert size in case of adenovirus vectors is

  1. 6-8 kb
  2. 4-6 kb
  3. 10-12 kb
  4. 20 kb

Answer : 1

Question 8 : SV 40 vectors, in which late region replacements are done, require

  1. COS cell line
  2. SV 40 helper virus
  3. pBR 322 vector
  4. none of these

Answer : 2

Question 9 : Which of the following(s) is/are the most commonly used markers in yeast?

  1. His 3
  2. Ura 3
  3. Trp 1
  4. All of these

Answer : 4

Question 10 : Which of the following are produced in high copy number?

  1. YEp
  2. YRp
  3. Both (1) and (2)
  4. YCp

Answer : 3

Question 11 : Cos cell line is

  1. cohesive end site of phage λ
  2. derivative of permissive CV-1 monkey cell line
  3. cohesive initial site of phage λ
  4. derivative of non-permissive CV-1 monkey cell line

Answer : 2

Question 12 : Lytic infection of SV 40 does not occur in

  1. permissive cells
  2. non permissive cells
  3. both (1) and (2)
  4. none of these

Answer : 2

Question 13 : Moloney-Mu-leukemia virus is an example of

  1. Vaccinia virus
  2. Baculo virus
  3. Retro virus
  4. none of these

Answer : 3

Question 14 : Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

  1. Allows transformation by using crossing over
  2. Has a origin of replication and rep genes
  3. Carries autonomously replicating sequence
  4. Carries centromere and telomere sequences

Answer : 1

Question 15 : The size of the genome of SV 40 is

  1. 7.2 kb
  2. 69 kb
  3. 5.2 kb
  4. 110 kb

Answer : 3

Question 16 : Cloning genes in the yeast offer which of following advantage(s)?

  1. Over production of proteins of commercial value
  2. Ability to glycosylate proteins during secretion
  3. Ability to clone large piece of DNA
  4. All of the above

Answer : 4

Question 17 : Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

  1. Allows transformation by using crossing over
  2. Has a origin of replication and rep genes
  3. Carries autonomously replicating sequence
  4. Carries centromere and telomere sequences

Answer : 2

Question 18 : Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

  1. Contains centromeric sequences
  2. Segregates during meiosis and mitosis
  3. Functions as true chromosome
  4. All of the above

Answer : 4

Question 19 : Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

  1. Vaccinia virus
  2. Baculo virus
  3. Papilloma virus
  4. Retrovirus

Answer : 2

Question 20 : Polyhedrin protein is

  1. non essential for baculo virus replication
  2. major component of protein matrix in which viruses are embedded
  3. both (1) and (2)
  4. essential for baculo virus replication

Answer : 3

Question 21 : Which of the following is/are commonly used promoter of S. cerevisiae?

  1. Galactokinase (GAL 1)
  2. Alcohol dehydrogenase II (ADH 2)
  3. Phosphoglycerate kinase (PGK)
  4. All of the above

Answer : 4

Question 22 : Maximum amount of foreign DNA introduced into SV 40 viruses is

  1. 10 kb
  2. 5 kb
  3. 2.5 kb
  4. 1 kb

Answer : 3

Question 23 : Recombinant SV40 viruses introduce the foreign DNA into cells

  1. with the DNA mediated transfection
  2. without the DNA mediated transfection
  3. with the RNA mediated transfection
  4. without the RNA mediated transfection

Answer : 2

Question 24 : Which one of the following is the most stable vector?

  1. YEp
  2. YRp
  3. YAC
  4. YIp

Answer : 3

Question 25 : SV 40 vectors, in which early region replacements are carried out, require

  1. COS cell line
  2. SV 40 helper virus
  3. pBR 322 vector
  4. none of these

Answer : 1

Question 26 : Which of the following histone is not present in SV40 virus?

  1. H1
  2. H2A
  3. H3
  4. H4

Answer : 1

Question 27 : Autonomously replicating sequence is not present in

  1. YIp
  2. YRp
  3. YCp
  4. YAC

Answer : 1

Question 28 : In case of Baculoviruses, the foreign gene

  1. is inserted downstream of polyhedrin promoter
  2. is inserted downstream of viral origin of replication
  3. is inserted between the polyhedrin promoter and downstream sequences
  4. is inserted randomly in viral genome

Answer : 3

Question 29 : α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

  1. His 3
  2. Lys 2
  3. Ura 3
  4. Trp 1

Answer : 2

Question 30 : Yeast episomal plasmids (YEp) were constructed using

  1. 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
  2. 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
  3. 12μm yeast plasmid and E coli vector pMB 9
  4. 12μm yeast plasmid and E coli vector pBR 322

Answer : 1

Question 31 : Yeast Replicating plasmids (YRp) carry

  1. centromere
  2. telomere
  3. autonomously replicating sequence
  4. both (1) and (3)

Answer : 3

Question 32 : Which of the following viruses are used as vectors for transferring genes to animal cells?

  1. SV 40 virus
  2. Bovine papilloma virus (BVP)
  3. Retroviruses
  4. All of these

Answer : 4

Question 33 : Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

  1. transcriptional regulators and are essential for viral replication
  2. not necessary for viral replication
  3. coding for virus coat protein
  4. important for packaging of viral DNA into viral head

Answer : 1

Question 34 : Retroviruses have advantage for being used as vector for animal cells because

  1. they cover a wide host range
  2. infection does not lead to cell death, infected cells produce virus over an indefinite period
  3. viral gene expression is driven by strong promoters
  4. all of the above

Answer : 4

Question 35 : Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

  1. episomally only
  2. integrated only
  3. either episomally or integrated at high copy number
  4. either episomally or integrated at low copy number

Answer : 3