Multiple Choice Questions and Answers on Vectors Use for Animal Cell Culture
Question 1 : 5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is
- His 3
- Lys 2
- Ura 3
- Trp 1
Answer : 3
Question 2 : Which of the following promoters is repressed in presence of glucose?
- Galactokinase (GAL 1)
- Acid phosphatase (PHOS)
- Both (1) and (2)
- Phosphoglycerate kinase (PGK)
Answer : 1
Question 3 : Which of the following can be used for reducing the size of gene inserts containing introns?
- Vaccinia virus
- Baculo virus
- Retro virus
- none of these
Answer : 3
Question 4 : Bovine papilloma virus has a circular double stranded DNA genome of about
- 2.5 kb
- 5.2 kb
- 7.9 kb
- 9.5 kb
Answer : 3
Question 5 : Retroviruses consist of
- Ds DNA genome
- Ss DNA genome
- RNA genome
- cDNA genome
Answer : 3
Question 6 : YAC vectors can be used to obtain artificial chromosomes because it carries
- centromere
- telomere
- autonomously replicating sequence
- all of these
Answer : 4
Question 7 : Maximum insert size in case of adenovirus vectors is
- 6-8 kb
- 4-6 kb
- 10-12 kb
- 20 kb
Answer : 1
Question 8 : SV 40 vectors, in which late region replacements are done, require
- COS cell line
- SV 40 helper virus
- pBR 322 vector
- none of these
Answer : 2
Question 9 : Which of the following(s) is/are the most commonly used markers in yeast?
- His 3
- Ura 3
- Trp 1
- All of these
Answer : 4
Question 10 : Which of the following are produced in high copy number?
- YEp
- YRp
- Both (1) and (2)
- YCp
Answer : 3
Question 11 : Cos cell line is
- cohesive end site of phage λ
- derivative of permissive CV-1 monkey cell line
- cohesive initial site of phage λ
- derivative of non-permissive CV-1 monkey cell line
Answer : 2
Question 12 : Lytic infection of SV 40 does not occur in
- permissive cells
- non permissive cells
- both (1) and (2)
- none of these
Answer : 2
Question 13 : Moloney-Mu-leukemia virus is an example of
- Vaccinia virus
- Baculo virus
- Retro virus
- none of these
Answer : 3
Question 14 : Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
- Allows transformation by using crossing over
- Has a origin of replication and rep genes
- Carries autonomously replicating sequence
- Carries centromere and telomere sequences
Answer : 1
Question 15 : The size of the genome of SV 40 is
- 7.2 kb
- 69 kb
- 5.2 kb
- 110 kb
Answer : 3
Question 16 : Cloning genes in the yeast offer which of following advantage(s)?
- Over production of proteins of commercial value
- Ability to glycosylate proteins during secretion
- Ability to clone large piece of DNA
- All of the above
Answer : 4
Question 17 : Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
- Allows transformation by using crossing over
- Has a origin of replication and rep genes
- Carries autonomously replicating sequence
- Carries centromere and telomere sequences
Answer : 2
Question 18 : Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
- Contains centromeric sequences
- Segregates during meiosis and mitosis
- Functions as true chromosome
- All of the above
Answer : 4
Question 19 : Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of
- Vaccinia virus
- Baculo virus
- Papilloma virus
- Retrovirus
Answer : 2
Question 20 : Polyhedrin protein is
- non essential for baculo virus replication
- major component of protein matrix in which viruses are embedded
- both (1) and (2)
- essential for baculo virus replication
Answer : 3
Question 21 : Which of the following is/are commonly used promoter of S. cerevisiae?
- Galactokinase (GAL 1)
- Alcohol dehydrogenase II (ADH 2)
- Phosphoglycerate kinase (PGK)
- All of the above
Answer : 4
Question 22 : Maximum amount of foreign DNA introduced into SV 40 viruses is
- 10 kb
- 5 kb
- 2.5 kb
- 1 kb
Answer : 3
Question 23 : Recombinant SV40 viruses introduce the foreign DNA into cells
- with the DNA mediated transfection
- without the DNA mediated transfection
- with the RNA mediated transfection
- without the RNA mediated transfection
Answer : 2
Question 24 : Which one of the following is the most stable vector?
- YEp
- YRp
- YAC
- YIp
Answer : 3
Question 25 : SV 40 vectors, in which early region replacements are carried out, require
- COS cell line
- SV 40 helper virus
- pBR 322 vector
- none of these
Answer : 1
Question 26 : Which of the following histone is not present in SV40 virus?
- H1
- H2A
- H3
- H4
Answer : 1
Question 27 : Autonomously replicating sequence is not present in
- YIp
- YRp
- YCp
- YAC
Answer : 1
Question 28 : In case of Baculoviruses, the foreign gene
- is inserted downstream of polyhedrin promoter
- is inserted downstream of viral origin of replication
- is inserted between the polyhedrin promoter and downstream sequences
- is inserted randomly in viral genome
Answer : 3
Question 29 : α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is
- His 3
- Lys 2
- Ura 3
- Trp 1
Answer : 2
Question 30 : Yeast episomal plasmids (YEp) were constructed using
- 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
- 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
- 12μm yeast plasmid and E coli vector pMB 9
- 12μm yeast plasmid and E coli vector pBR 322
Answer : 1
Question 31 : Yeast Replicating plasmids (YRp) carry
- centromere
- telomere
- autonomously replicating sequence
- both (1) and (3)
Answer : 3
Question 32 : Which of the following viruses are used as vectors for transferring genes to animal cells?
- SV 40 virus
- Bovine papilloma virus (BVP)
- Retroviruses
- All of these
Answer : 4
Question 33 : Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
- transcriptional regulators and are essential for viral replication
- not necessary for viral replication
- coding for virus coat protein
- important for packaging of viral DNA into viral head
Answer : 1
Question 34 : Retroviruses have advantage for being used as vector for animal cells because
- they cover a wide host range
- infection does not lead to cell death, infected cells produce virus over an indefinite period
- viral gene expression is driven by strong promoters
- all of the above
Answer : 4
Question 35 : Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
- episomally only
- integrated only
- either episomally or integrated at high copy number
- either episomally or integrated at low copy number
Answer : 3